| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046243 |
| Chromosome: | chromosome 4 |
| Location: | 1258475 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215050 | CHYB,CHYB1,CBH1 | Beta-carotene hydroxylase, putative chloroplast precursor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGTAAACGGCGGGGCGCGAGGCCACAT |
| Internal bar code: | CGTACTCCAGCGCATATTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 98.9791 |
| LEAP-Seq n confirming: | 12410 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGTGCATGCATTGAGAG |
| Suggested primer 2: | GTGGACATTGGCACACTCAC |