| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046783 |
| Chromosome: | chromosome 7 |
| Location: | 2485659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329300 | MSC1 | Mechanosensitive ion channel; (1 of 1) PTHR30221 - SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACATCCGCATCCATGACGCAGGAATTT |
| Internal bar code: | AACGTGGAAGGTGTCCTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 99.6944 |
| LEAP-Seq n confirming: | 4893 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACATGCGTTATTGGCGTGT |
| Suggested primer 2: | GCCTTCATCACCGTGTAGGT |