| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047123 |
| Chromosome: | chromosome 12 |
| Location: | 3818529 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515350 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACGCTGTGGGGCAACATGGAAGATTGCA |
| Internal bar code: | CTGTTGGCTACCGCCGCGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 81.2092 |
| LEAP-Seq n confirming: | 7455 |
| LEAP-Seq n nonconfirming: | 1725 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGATGCCCAAGGTATGTT |
| Suggested primer 2: | CAACCACTCACAAACATGCC |