| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047720 |
| Chromosome: | chromosome 11 |
| Location: | 3156215 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479300 | SRH20 | SNF2-related DNA/RNA helicase; (1 of 1) K15083 - DNA repair protein RAD16 (RAD16) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCTGCGTGGCACGCGCGGGCAGGACAG |
| Internal bar code: | CCGGGTGTGGGCTCCGTTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 99.7214 |
| LEAP-Seq n confirming: | 4653 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGGTCTTGAGAGGCAGT |
| Suggested primer 2: | GGAGTGGACAAAAGGGAACA |