| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048377 |
| Chromosome: | chromosome 11 |
| Location: | 391211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467579 | CSC6 | Chlamydomonadale-specific family C protein | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGAACCAATTGGACTGTTGGACTGGTG |
| Internal bar code: | GTGGACCAGCGGCATGTTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1131 |
| LEAP-Seq percent confirming: | 98.0354 |
| LEAP-Seq n confirming: | 998 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAGTGAAAGCTGGAATA |
| Suggested primer 2: | GACGGCATGTCACCCTTAGT |