| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048892 |
| Chromosome: | chromosome 3 |
| Location: | 2885700 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162800 | LCI1 | Low-CO2-inducible membrane protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAAACTTGATGCTTGCATGTGTGCAAAG |
| Internal bar code: | GGTGGGGGGGACGTCATCGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 99.5719 |
| LEAP-Seq n confirming: | 2326 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTTGCGCAATAGAAGAG |
| Suggested primer 2: | TTTGTGCATCGAAACTGCTC |