| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049225 |
| Chromosome: | chromosome 12 |
| Location: | 9457088 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539650 | (1 of 1) PF00530//PF08016 - Scavenger receptor cysteine-rich domain (SRCR) // Polycystin cation channel (PKD_channel) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGCCGCCCCGCTCTGCTTCATGACCCG |
| Internal bar code: | TCGCGTGCGCAAGGAGGAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 99.6283 |
| LEAP-Seq n confirming: | 268 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCTTCCGTATCCTCCA |
| Suggested primer 2: | CGTTGTACAGCTTGCCGTTA |