| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050166 |
| Chromosome: | chromosome 10 |
| Location: | 4807804 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453900 | CYN71 | (1 of 1) K12736 - peptidylprolyl isomerase domain and WD repeat-containing protein 1 [EC:5.2.1.8] (PPWD1); Cyclophilin 71 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTCGGAAGCTGAGGGCTCAGGGAGGTG |
| Internal bar code: | AAGCTCTAATTAAATACGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 99.6951 |
| LEAP-Seq n confirming: | 1962 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGGATGTGTAGGCCAGG |
| Suggested primer 2: | CTCCTCGCCGACCTAGTATG |