| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050177 |
| Chromosome: | chromosome 13 |
| Location: | 3277132 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586050 | (1 of 1) PTHR13833:SF49 - NHL REPEAT-CONTAINING PROTEIN 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACAAACAATTCAGAACTCTCAAACGTT |
| Internal bar code: | GCGCGCTGACGCAACTGTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 98.7552 |
| LEAP-Seq n confirming: | 476 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGCATCAACGGGTCTTC |
| Suggested primer 2: | ATGTGTGGGCAAGGAGATTC |