| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050384 |
| Chromosome: | chromosome 6 |
| Location: | 7250707 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298300 | PPR2,MRL1 | rbcL mRNA stabilization factor; (1 of 2) PF01535//PF13812 - PPR repeat (PPR) // Pentatricopeptide repeat domain (PPR_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTACACGAGTGCATGCGGGTATAATGG |
| Internal bar code: | CGGTAGAGCAAGGACGTGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1051 |
| LEAP-Seq percent confirming: | 99.4376 |
| LEAP-Seq n confirming: | 3006 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCGAAAGAAGCAGTGGG |
| Suggested primer 2: | GCAGCTTTTCTGGTCTGGTC |