| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050597 |
| Chromosome: | chromosome 4 |
| Location: | 2254525 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220200 | (1 of 1) PTHR16254:SF13 - K(+) EFFLUX ANTIPORTER 1, CHLOROPLASTIC-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTACGCACTGCTGCTAAGCATGGGTCTA |
| Internal bar code: | CTGGAAATGTGCGTATCCCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 99.3475 |
| LEAP-Seq n confirming: | 4263 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGACTTGTGTGCTTTCC |
| Suggested primer 2: | GCTAGCTGGAGTGGGAGTTG |