| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051057 |
| Chromosome: | chromosome 4 |
| Location: | 3083422 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225400 | (1 of 79) IPR011992 - EF-hand domain pair | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCAACAAGCCCAGACTTGAATCTACGGT |
| Internal bar code: | AGGTGCACTAAGTATATTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1126 |
| LEAP-Seq percent confirming: | 99.829 |
| LEAP-Seq n confirming: | 7004 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACATAAGGAACGGGTTG |
| Suggested primer 2: | GCGACTGCTAGATAGGCACC |