| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051225 |
| Chromosome: | chromosome_7 |
| Location: | 1245510 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g321400 | FAP113 | Flagellar Associated Protein | sense | 5'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGAATATAGACAGCCAGATACACCTACCGA |
| Internal bar code: | ATGGCACGCCTACTCCCTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1160 |
| LEAP-Seq percent confirming: | 99.0577 |
| LEAP-Seq n confirming: | 5151 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTAATCGTGCTCGTCAG |
| Suggested primer 2: | AAAGCCCCACGCCTAGTAAT |