Insertion junction: LMJ.RY0402.051439_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.051439
Chromosome:chromosome 6
Location:1101531
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g256700 (1 of 1) IPR014001//IPR026127//IPR027417 - Helicase superfamily 1/2, ATP-binding domain // Probable RNA helicase SDE3 // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGCGACGCGCGGCCACCCTACGCAGTGG
Internal bar code:CCCCGATACGCTGTGGGTGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:877
LEAP-Seq percent confirming:77.169
LEAP-Seq n confirming:169
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTCATCCTCAGGGCTTT
Suggested primer 2:CGACCGATGTTCCTGTAGGT