| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051461 |
| Chromosome: | chromosome 10 |
| Location: | 1015176 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425050 | ROC59,ROC28 | (1 of 1) K11807 - WD and tetratricopeptide repeats protein 1 (WDTC1); Rhythm Of Chloroplast 59 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCATTGTAAGGATCACTCGGGAACGTTG |
| Internal bar code: | TGACGCGTGATAATTACTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTAAGCTGCGATGTAGGC |
| Suggested primer 2: | ACGTGCATTCTTTCTTCGCT |