| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051500 |
| Chromosome: | chromosome 10 |
| Location: | 1403460 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAAGACAGGCGGGCATCGGCAGGCTGC |
| Internal bar code: | GGGGGAAGTCTTAAGGCTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 74.2739 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGTCTCAGTTTGCCAGC |
| Suggested primer 2: | TAGAAGCATGTTGGCACCTG |