Insertion junction: LMJ.RY0402.051781_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.051781
Chromosome:chromosome 11
Location:980672
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467665 (1 of 1) IPR002909//IPR006626//IPR011050//IPR014756//IPR019316 - IPT domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Immunoglobulin E-set // G8 domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTGGGCGAGTGGCATGGGGCAGGGGCGG
Internal bar code:AGCTCCTTCGCAGCGTAAACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:99.548
LEAP-Seq n confirming:1982
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTCCTCATGCTGCTCGT
Suggested primer 2:GCGAGTGGGATGATGATTG