Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.051955 |
Chromosome: | chromosome_5 |
Location: | 2240452 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g234661 | BCS1 | Ubiquinol:cytochrome c oxidoreductase biogenesis factor | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAGATTAACAAGCACGTGCAGGTGAGAGGC |
Internal bar code: | CGGATCGTTCCGTAGGCGGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 638 |
LEAP-Seq percent confirming: | 99.839 |
LEAP-Seq n confirming: | 620 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTACGTGATCTTGGTGGCCT |
Suggested primer 2: | TGCTTGTCACAATGGTGGAT |