| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052289 |
| Chromosome: | chromosome 2 |
| Location: | 2530623 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092500 | UPF1 | (1 of 1) PTHR10887//PTHR10887:SF378 - DNA2/NAM7 HELICASE FAMILY // REGULATOR OF NONSENSE TRANSCRIPTS 1 HOMOLOG; UPF1-like RNA helicase, NMD protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTACCGCGCCGTAATCCTCTCGCCGTC |
| Internal bar code: | CTAACGCTGCTTGACCGGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 99.8133 |
| LEAP-Seq n confirming: | 4278 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGTGCACATATGCTTTT |
| Suggested primer 2: | GCACCTCCTTGTTCTTGCTC |