Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.052322 |
Chromosome: | chromosome_13 |
Location: | 2255658 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g578750 | TBA1 | PsbA translation factor | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCAGGTCGCACTGCACTGCACGCATTGCTT |
Internal bar code: | GAGGACTGATATTAATGCCCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1047 |
LEAP-Seq percent confirming: | 98.8551 |
LEAP-Seq n confirming: | 2504 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTACTCGTGGAAGGCGGTAA |
Suggested primer 2: | GGCAATCCGTCAAAACCTAA |