Insertion junction: LMJ.RY0402.052322_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.052322
Chromosome:chromosome_13
Location:2255658
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g578750 TBA1 PsbA translation factor sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TCAGGTCGCACTGCACTGCACGCATTGCTT
Internal bar code:GAGGACTGATATTAATGCCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1047
LEAP-Seq percent confirming:98.8551
LEAP-Seq n confirming:2504
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTCGTGGAAGGCGGTAA
Suggested primer 2:GGCAATCCGTCAAAACCTAA