| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052322 |
| Chromosome: | chromosome 13 |
| Location: | 2255667 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578750 | TBA1 | (1 of 1) K00207 - dihydropyrimidine dehydrogenase (NADP+) (DPYD); Translation factor for chloroplast psbA mRNA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCCCGCTGGCTAGTTTTCCCCGCTGG |
| Internal bar code: | TGGAACGATTTGAGGGTCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 99.5743 |
| LEAP-Seq n confirming: | 7485 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTCGTGGAAGGCGGTAA |
| Suggested primer 2: | GGCAATCCGTCAAAACCTAA |