| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052381 |
| Chromosome: | chromosome_4 |
| Location: | 2954533 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g224800 | VAMP74,VAM4 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III) | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTACATTGCATTCTCACTTTCCAACAATG |
| Internal bar code: | CTGGGACGAGGTAGACGGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.2893 |
| LEAP-Seq n confirming: | 3912 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTCAAGTAAACGGCTC |
| Suggested primer 2: | ACCTCTCGGAACATTCATGC |