| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052682 |
| Chromosome: | chromosome_4 |
| Location: | 674560 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g217951 | LCI33 | Low-CO2-inducible protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCACTTTGTAATATGAACCAGTTGCTCTT |
| Internal bar code: | CATGCGCGTGGGTTTGTTATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 99.4316 |
| LEAP-Seq n confirming: | 2274 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAGCCTGGACAACAGAG |
| Suggested primer 2: | ACCGTAATATTGCCGACTGC |