| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052695 |
| Chromosome: | chromosome 9 |
| Location: | 4589577 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396401 | SUMT1:COBA,UPM1 | S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase; (1 of 1) PTHR21091//PTHR21091:SF16 - METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED // SIROHEME SYNTHASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACCCGCGCCCACCAGCTGCGAGGCCAGC |
| Internal bar code: | CGGGTATCATACGGTCCGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGTAACAAGGAGGTGGC |
| Suggested primer 2: | ACCAGTAGCTCCCGGTTTCT |