Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.053337 |
Chromosome: | chromosome_12 |
Location: | 7634274 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g554850 | TRX7,TRXh1,TRXh2 | Thioredoxin h2, cytosolic | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACGCTACTGCCCTGTGCTCGGCGGCAGGC |
Internal bar code: | CGAGCCGACTCGATCAAAAGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 415 |
LEAP-Seq percent confirming: | 89.0625 |
LEAP-Seq n confirming: | 456 |
LEAP-Seq n nonconfirming: | 56 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGATGAGGCTCGGCTAGTG |
Suggested primer 2: | GGAATGTGATGTTGGGGTTC |