| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053552 |
| Chromosome: | chromosome 12 |
| Location: | 3803027 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515350 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCGAGCCCTAAGCCACCGTCCCCGGT |
| Internal bar code: | TGTGATTCGGAGGCCCCATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 96.0145 |
| LEAP-Seq n confirming: | 14840 |
| LEAP-Seq n nonconfirming: | 616 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTAACTAGCTGAGCCGCC |
| Suggested primer 2: | AGTACTGAAGCTGCCACCGT |