| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054407 |
| Chromosome: | chromosome 12 |
| Location: | 7287461 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557700 | (1 of 1) K01069 - hydroxyacylglutathione hydrolase (E3.1.2.6, gloB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAATGCACACTCGCGGGCTTACTGCAT |
| Internal bar code: | GCGTCGTCGATGGCAAGTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.3457 |
| LEAP-Seq n confirming: | 6833 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTCGACAAGTGTTCCCC |
| Suggested primer 2: | CGTTCTCCATATGAACGGCT |