| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.054596 |
| Chromosome: | chromosome 14 |
| Location: | 3519000 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630883 | (1 of 2) K03921 - acyl- (DESA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCATTGGGTAAGGGGACTCGCCGATCT |
| Internal bar code: | GCGCCCCGGTGTGATATCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 99.7096 |
| LEAP-Seq n confirming: | 5150 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGGTGAATGTGTTTCCT |
| Suggested primer 2: | CCGTTGACGCTGTGTCTCTA |