| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054839 |
| Chromosome: | chromosome 3 |
| Location: | 4559465 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176833 | NAP1,NAP,DII5 | (1 of 7) PF00022 - Actin (Actin); Novel actin protein 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTTTGCCAGCGTGACGGGTCGGCCGCG |
| Internal bar code: | GGCCTTCAAGGAGGGTTGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 99.6038 |
| LEAP-Seq n confirming: | 2011 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGTAGTCCAAAGCTCA |
| Suggested primer 2: | ACACCGCATCATGTTCGTAA |