| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054907 |
| Chromosome: | chromosome 11 |
| Location: | 388685 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467579 | CSC6 | Chlamydomonadale-specific family C protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGTGGTCCCCCGTTGAGCATTTAGCG |
| Internal bar code: | CAACTTTGTAAGTTCGCTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 648 |
| LEAP-Seq percent confirming: | 99.7183 |
| LEAP-Seq n confirming: | 1416 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACTGCTAGATAGGCACC |
| Suggested primer 2: | GGTAGCACGTTGTCCGTTTT |