| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054922 |
| Chromosome: | chromosome 12 |
| Location: | 5463292 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530650 | GLN2 | Glutamine synthetase; (1 of 2) PTHR20852//PTHR20852:SF57 - GLUTAMINE SYNTHETASE // GLUTAMINE SYNTHETASE 2 CYTOPLASMIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGGGACGTATTGTGCTGCCCAACCGCGT |
| Internal bar code: | TGCCGGGCCAGCGGCGATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 97.3726 |
| LEAP-Seq n confirming: | 4966 |
| LEAP-Seq n nonconfirming: | 134 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGGCAAAGAGAAAAGAC |
| Suggested primer 2: | TTATCCTTTCACCACAGGGC |