Gene: Cre12.g530650

Overview

Common name:GLN2
Defline:Glutamine synthetase; (1 of 2) PTHR20852//PTHR20852:SF57 - GLUTAMINE SYNTHETASE // GLUTAMINE SYNTHETASE 2 CYTOPLASMIC
Description:Glutamine synthetase (EC 6.3.1.2), chloroplast precursor (Glutamate--ammonia ligase) (GS2), CO2-responsive gene; identical to GLNA2_CHLRE cDNA [SwissProt Q42689), PMID: 8938407]; arranged head-to-head with another glutamine synthetase gene. GLN3; GLN2
Synonyms:g13061.t1
Chromosome:chromosome 12

Protein Localization

A plasmid for expressing Cre12.g530650 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre12.g530650-Venus-3xFLAG

Imaged strain: CSI_LW07B10

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

chloroplast/homogeneous/pyrenoid depleted

shapes in cytoplasm/a few small patches in cytoplasm

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

We did not determine any protein-protein interactions for this gene.

Mutant Strains

Insertion Junction Mutant Strain Insertion Junction Feature Confidence (%) Genome Version
CLIP2.003888_1 CLIP2.003888 intron 80 6.1
CLIP2.003888_2 CLIP2.003888 intron 80 6.1
LMJ.RY0402.054922_1 LMJ.RY0402.054922 intron 95 5.5
LMJ.RY0402.054922_2 LMJ.RY0402.054922 intron 95 5.5
LMJ.RY0402.120753_1 LMJ.RY0402.120753 3'UTR|outside_mRNA 58 5.5
LMJ.RY0402.160272_1 LMJ.RY0402.160272 3'UTR 73 5.5
LMJ.RY0402.212026_1 LMJ.RY0402.212026 CDS 73 5.5

Gene Annotations

PFAM:PF00120, PF03951
PANTHER: PTHR20852 PTHR20852:SF57
KEGG_ec:EC:6.3.1.2
KEGG_orthology:K01915
KOG:KOG0683
Gene ontology terms::GO:0004356, GO:0006542, GO:0006807
Best arabidopsis TAIR name:AT5G16570.1
Best arabidopsis TAIR symbol:GLN1;4
Best arabidopsis TAIR defline:glutamine synthetase 1;4
Phytozome Locus Page

Phenotypes

Our phenotype screens did not find statistically significant results for this gene.