| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120753 |
| Chromosome: | chromosome 12 |
| Location: | 5465246 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530650 | GLN2 | Glutamine synthetase; (1 of 2) PTHR20852//PTHR20852:SF57 - GLUTAMINE SYNTHETASE // GLUTAMINE SYNTHETASE 2 CYTOPLASMIC | 3'UTR|outside_mRNA |
| Cre12.g530700 | (1 of 1) K03126 - transcription initiation factor TFIID subunit 12 (TAF12) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTATGAGAGGTGCGGTGCGGTCACAGTC |
| Internal bar code: | CTGTGCCATCCATCCGGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 61.5955 |
| LEAP-Seq n confirming: | 1745 |
| LEAP-Seq n nonconfirming: | 1088 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCGGATGTGAGCTGTGA |
| Suggested primer 2: | GGAGTTCTACACCGAGTGGG |