| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212026 |
| Chromosome: | chromosome 12 |
| Location: | 5463025 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530650 | GLN2 | Glutamine synthetase; (1 of 2) PTHR20852//PTHR20852:SF57 - GLUTAMINE SYNTHETASE // GLUTAMINE SYNTHETASE 2 CYTOPLASMIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGATGGCAACGAGGGCAAGCCTGAGAA |
| Internal bar code: | GGGGGCATGGCTGGCACGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 99.6256 |
| LEAP-Seq n confirming: | 6919 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTTTTGACAGCCAACC |
| Suggested primer 2: | GCGTGGCAAAGAGAAAAGAC |