| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055477 |
| Chromosome: | chromosome 1 |
| Location: | 4129602 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027150 | CPLD46,HEL5 | Conserved in the plant lineage and diatoms; (1 of 2) PTHR24031//PTHR24031:SF249 - RNA HELICASE // ATP-DEPENDENT DNA HELICASE RECG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCATGCGCTGAGCAGGCCGCCTCTGGAT |
| Internal bar code: | GGCCCCTGCGCGCGCGGTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 93.7161 |
| LEAP-Seq n confirming: | 865 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGCTGACGTTCTAGAGG |
| Suggested primer 2: | TAGGACATGAGCAGGAGGCT |