| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055562 |
| Chromosome: | chromosome 3 |
| Location: | 1939783 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155450 | (1 of 3) PF01167 - Tub family (Tub) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTAGCGGACCCACTCCTGCGGCCGTAGG |
| Internal bar code: | AAACCGTGTGGCTTTGAGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 99.8149 |
| LEAP-Seq n confirming: | 2157 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCAACCCCTGTGTGCTC |
| Suggested primer 2: | CTCATCCTCTGGAGTGAGGC |