Insertion junction: LMJ.RY0402.055589_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.055589
Chromosome:chromosome 13
Location:3273472
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g586050 (1 of 1) PTHR13833:SF49 - NHL REPEAT-CONTAINING PROTEIN 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGGCGAGTTGAGCGGCTGTGTGCAGGCA
Internal bar code:CGAGGCGACCAAGCAGGGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:75.576
LEAP-Seq n confirming:19157
LEAP-Seq n nonconfirming:6191
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:TGAACAGGTTGTCGCTGAAG
Suggested primer 2:GGGGGCATCATAAGTTGCTA