| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055768 |
| Chromosome: | chromosome 13 |
| Location: | 1667207 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574000 | GEF1,CLV1 | Voltage-gated chloride channel; (1 of 1) PTHR11689:SF59 - CHLORIDE TRANSPORT PROTEIN 6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGCTGGCTCCCACCTGGCTGCTCATGA |
| Internal bar code: | CGTGGTTGGAAAGACCGTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 96.8074 |
| LEAP-Seq n confirming: | 1789 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCGGTAAGAGGGAAGGT |
| Suggested primer 2: | CCATCTGCCTCAGCTCCTAC |