| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.056064 |
| Chromosome: | chromosome_1 |
| Location: | 844894 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g004350 | ALIX,HLM1,ROC77 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGGCCGATGGCCTTGGCAATGCGGCGGG |
| Internal bar code: | CAATCCGACGGCTGGCAGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 96.0714 |
| LEAP-Seq n confirming: | 269 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTGTGGACACTAATCCC |
| Suggested primer 2: | AGGCAAGCAAGAATGACGAT |