Insertion junction: LMJ.RY0402.056697_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.056697
Chromosome:chromosome 3
Location:1429086
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTCCTCCACCTCCAAGATGGCGGGCCC
Internal bar code:GTGCGTTATAAAGTGCGGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:417
LEAP-Seq percent confirming:99.5413
LEAP-Seq n confirming:434
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAGTTGTAAGCGGTGTCG
Suggested primer 2:CGTGTGTAACGCTTTGTGCT