| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.057181 |
| Chromosome: | chromosome 12 |
| Location: | 1175015 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489900 | CYG54 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCACCTTCGGCCGACAAGCAGCTTGTT |
| Internal bar code: | GCGCGCCAGATTAGCCTGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 99.7324 |
| LEAP-Seq n confirming: | 4099 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGAGGCCACTCCCATAC |
| Suggested primer 2: | CTGTAGCAAGCCCCATGTTT |