| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057309 |
| Chromosome: | chromosome 7 |
| Location: | 3487038 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335750 | IFT88 | (1 of 1) K16474 - intraflagellar transport protein 88 (IFT88); Intraflagellar Transport Protein 88 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACAGTGGCACCACCGGCCCCCTGCGACG |
| Internal bar code: | AAAAAGTACCCAAAGGGTTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 99.7178 |
| LEAP-Seq n confirming: | 2827 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTTTGAGCTAATTGGG |
| Suggested primer 2: | TTTGGAAAGGTGCTGAGCTT |