| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058040 |
| Chromosome: | chromosome 3 |
| Location: | 4176603 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173800 | PDXK1,SOS4,PDX2 | (1 of 1) 2.7.1.35 - Pyridoxal kinase / Vitamin B6 kinase; Pyridoxal kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGGTCACAACCCTAGCCGTGCGCGACC |
| Internal bar code: | GCCGTAGCTTAATTTCTTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 66.828 |
| LEAP-Seq n confirming: | 3175 |
| LEAP-Seq n nonconfirming: | 1576 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCGCACTCCTAAAAGC |
| Suggested primer 2: | GCTAGAGAGCTGCAGGGAGA |