Insertion junction: LMJ.RY0402.058274_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.058274
Chromosome:chromosome 4
Location:1922878
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g218150 PPH1,PPP18 Thylakoid phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCAAGCCGCCCATCAAGACCGTGGCGGG
Internal bar code:AGAGGACACGTGGGAACCCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:749
LEAP-Seq percent confirming:99.2611
LEAP-Seq n confirming:1612
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCTTGTGGTTCCGACCT
Suggested primer 2:GGACGCTTGCTAATTTCTGC