| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.058359 |
| Chromosome: | chromosome 9 |
| Location: | 5622118 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400997 | (1 of 7) IPR001024 - PLAT/LH2 domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACGGTCGCGGAGGGTCTAAAATGCTTT |
| Internal bar code: | GCACCAATAAATCAGGCGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACCAAACGGAACTATGC |
| Suggested primer 2: | GTAGGCTTGGTGCATGGTTT |