| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058653 |
| Chromosome: | chromosome 9 |
| Location: | 3508251 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389763 | HPAT4 | Hydroxyproline O-arabinosyl transferase 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCTGGTCGGGATCACGGCCTGACCCC |
| Internal bar code: | CTGAATGGGACCGGATAGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 99.3275 |
| LEAP-Seq n confirming: | 15508 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTCAGTTCCAGTCGTTT |
| Suggested primer 2: | TCTGGCATTACTCATGCTGC |