| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.058679 |
| Chromosome: | chromosome 1 |
| Location: | 3856152 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024950 | (1 of 1) PTHR12444//PTHR12444:SF0 - UNCHARACTERIZED // PROTEIN EFR3 HOMOLOG CMP44E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGCTTGTCAAGCTCTGAGCATGTGCGC |
| Internal bar code: | ATTGCCGGTTCTGTTTTATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 98.9948 |
| LEAP-Seq n confirming: | 3841 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCCTCGTCTGCACAACA |
| Suggested primer 2: | TGTGATCCCTACAGCCAACA |