| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.058754 |
| Chromosome: | chromosome 10 |
| Location: | 3447094 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444700 | SBE3,SBE2b | (1 of 3) 2.4.1.18 - 1,4-alpha-glucan branching enzyme / Glycogen branching enzyme; Starch Branching Enzyme 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGCGTGTGTGGCGGGCACGGGGTCCC |
| Internal bar code: | ATTCGCATCTGCGAAGGCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 99.4049 |
| LEAP-Seq n confirming: | 10857 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACGGTGGGCATAGACAC |
| Suggested primer 2: | TGCACGAGGGTAAGGGTAAG |