| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.058905 |
| Chromosome: | chromosome 9 |
| Location: | 2886337 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388372 | RAT2,OPR39 | tscA RNA stabilization factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGGGCCCGACTGCTCCTCGCCACGAC |
| Internal bar code: | GTGGTCGTCGGGAATAGGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 20.7407 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 214 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCCAGCTCTTTCAGTTC |
| Suggested primer 2: | TTGCGTGCAAACCAATACAT |