Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.059490 |
Chromosome: | chromosome 3 |
Location: | 5487198 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g185550 | SEBP1,SBP1 | (1 of 2) 3.1.3.37 - Sedoheptulose-bisphosphatase / Sedoheptulose-1,7-bisphosphatase; Sedoheptulose-1%252C7-bisphosphatase, chloroplastic | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAAAAGCAGTCAGCCAACACTCATCGT |
Internal bar code: | CAGATGAGTCAAGTAATAAGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 862 |
LEAP-Seq percent confirming: | 99.2978 |
LEAP-Seq n confirming: | 2828 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATAGCTTAGCATCCAGCCGA |
Suggested primer 2: | GGGAGAAATGAAGCAATGGA |