Insertion junction: LMJ.RY0402.059490_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.059490
Chromosome:chromosome 3
Location:5487198
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g185550 SEBP1,SBP1 (1 of 2) 3.1.3.37 - Sedoheptulose-bisphosphatase / Sedoheptulose-1,7-bisphosphatase; Sedoheptulose-1%252C7-bisphosphatase, chloroplastic 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAAAAGCAGTCAGCCAACACTCATCGT
Internal bar code:CAGATGAGTCAAGTAATAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:862
LEAP-Seq percent confirming:99.2978
LEAP-Seq n confirming:2828
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGCTTAGCATCCAGCCGA
Suggested primer 2:GGGAGAAATGAAGCAATGGA